CytoCypher has built a software package that includes everything that is needed for the analysis of your data. With smart algorithms it is now possible to upload your files, automatically reject transients with artefacts and export your data to excel or CSV
CytoSolver uses the same important parameters found in IonWizard’s core analysis, which has been the standard for the past 15 years, but with improvements where they were desired. For example, uploading multiple files and scoring and detection of arrhythmias. To see the improvements compared with IonWizard’s core analysis, click here
We also provide the option to store your files and analysis in the CytoSolver online database. Your data will be secured and protected at all times, following the best European practices of data protection and regulation. This way all your files are stored safely and you can access them on every device that has an internet connection.
There are some requirements for the data files that can be analysed with CytoSolver. To find out what these are, click here
CytoSolver has everything that IonWizard offers, but with some extra’s. The baseline detection and accuracy of relaxation parameters have been improved and we added some new features. It is now possible to upload 20 files simultaneously, automatically reject transients with artefacts, and you will be able to visualize your data on any computer, phone or tablet. Furthermore, the analysis results can be directly exported to CVS or an Excel file, with the option to combine all data in one sheet or in single sheets. This is just the beginning; many new features are waiting in the wings. For example,
we are working on machine learning algorithms that allow you to train the system to detect arrhythmias or other regularly occurring oddities.
We are looking for beta-users, click here to find out what we are looking for!
|Uploading multiple files.||-||+||With CytoSolver it is possible to upload 20 files simultaneously.|
|Automatic rejection of transients||-||+||A set of rules has been added to the program that automatically reject transients with artefact or empty data.|
|Baseline detection||- +||+||Even though IonWizard has baseline detection, we have improved this in CytoSolver.|
|Accuracy of relaxation parameters||- +||+||With single and double exponential fits, accuracy has improved.|
|Detection and scoring of arrhythmic contractions||-||+||It will detect multiple peaks in the contractility transients, or when the cell skips a beat.|
|Automated Export of your analysis.||-||+||In CytoSolver you can easily export to a CVS of an Excel file. No need for manual copying and pasting.|
|Cloud based||-||+||CytoSolver is a cloud based software program, so it is possible to access your files from every device with an internet connection.|
CytoSolver can be used on files collected with IonWizard data acquisition software. To make the best use of CytoSolver it is important that the data is collected with the following requirements. The most important part is to make use of the ‘epoch’ concept of IonWizard. CytoSolver considers separate epochs (the separation is the red line that can be seen when opening the files in IonWizard) as different cells or treatments. Therefore, you should use epochs to structure your files during acquisition.
The following data types can be analysed: Sarcomere length, cell length, force and fluorescence data (all types: single and dual excitation, dual emission, interpolated numerator). Each transient should have at least 50 data points, leading to the following table:
|Sampling frequency:||Pacing frequency:|
|500 Hz||¼ Hz - 10 Hz|
|250 Hz||¼ Hz - 5 Hz|
|100 Hz||¼ Hz - 2 Hz|
You can vary the stimulation frequency during your experiment but be sure you start a new epoch.
Text marks can be inserted while acquiring data or after finishing the experiment. They help CytoSolver to correctly analyse the data. The list below describes the action that is automatically taken by CytoSolver while analysing the data.
Delete: ignores this epoch in the analysis
Begin: defines and replaces the begin of the interval (Begin- and End must be used together)
End: defines and replaces the end of the interval
Background: Identifies an epoch as a background (calcium)
Tip: to make things easier you can create ‘shortcut keys’ for each text mark. Shortcut keys can only be used while acquiring data and can be assigned in IonWizard under the Collect tab > Mark text...
|Remark||Indicates remarks made during data acquisition|
|NumberOfTransients||Indicates the number of transients analysed|
|Baseline||The pre-stimulation baseline value of the recorded signal|
|DepartureVelocity||The velocity at the start of deflection|
|DepartureVelocityTime||The time at the departure velocity relative to pacing mark|
|Peak||The value of the transient at its maximal deflection from baseline|
|Time to peak||The time at which peak occurs relative to the pacing mark|
|Peak Height||peak - baseline.|
|Percentage of shortening||100 x peak height / baseline|
|Times to % Peak||Times for the transient to reach a percent of the peak during the deflection phase of the transient|
|Times to % Baseline||Times for the transient to return a percent of the peak during the recovery phase of the transient|
|ReturnVelocity||The velocity at the start of recovery|
|ReturnVelocityTime||The time at the return velocity relative to pacing mark|
|Time Constants (tau)||The exponential time constants associated with recovery phases of the transients|
|R2(fit)||The goodness of fit of the indicated transient phase|
|Arrhythmia||Indicates if there is an arrhythmia or not|
|Contraction escape||Indicates if there is an electrical stimulation but no transient|
|After Contraction||Indicates if there is an after contraction in the transient|
|After Contraction size||100 x after contraction peak height / transient peak height|
We have developed CytoSolver, a cloud-based program that accelerates and improves the analysis of calcium and contractility data from cardiac myocytes. We are proud to tell you that our software is ready to be tested and therefore we are looking for beta users. For the time being it is free and without any obligations!
The advantages of being a beta-user? You are among the first to benefit from our new platform. It will save you over 95% analysis time! You will also be able to suggest improvements for future versions so we can help you analyse your data even better.
There are a few conditions that must be met in order to become a beta user. First, our software analyses .ZPT files created by IonWizard, which means that your data acquisition must be done with IonOptix software (for extensive data requirements click here). Second, to make sure that you understand and use the software the way it is developed, we ask you to read our two-page user guide. At last, as it is beta testing, we would like to hear your feedback every time a file fails to analyse and only with your permission, we would like to get access to the file to find out why it has failed to analyse.
N.B. The CytoSolver analyses are validated based on mouse and rat myocyte data. For other species, please run comparisons and let us know if you run into problems.